Объем | 50 мкл |
Синонимы | Histone H2B type 1-L (Histone H2B.c) (H2B/c), HIST1H2BL, H2BFC |
Клональность | Polyclonal |
Организм | Human |
uniprot | Q99880 |
Иммуноген | Recombinant Human Histone H2B type 1-L protein (2-126AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IHC, Recommended dilution: IHC:1:200-1:500 |
Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Histone H2B type 1-L |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA860357EA01HU diluted at 1:300 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA860357EA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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