Объем | 100 мкг |
Синонимы | Indian hedgehog protein (IHH) (HHG-2) [Cleaved into: Indian hedgehog protein N-product, Indian hedgehog protein C-product], IHH |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q14623 |
Иммуноген | Recombinant Human Indian hedgehog protein (203-411AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA622779LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA622779LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA622779LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunocytochemistry analysis of human pancreatic tissue using CSB-PA622779LA01HU at dilution of 1:100
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IHC image of CSB-PA622779LA01HU diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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