| Объем | 50 мкг |
| Синонимы | Ribosomal biogenesis protein LAS1L (Protein LAS1 homolog), LAS1L |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9Y4W2 |
| Иммуноген | Recombinant Human Ribosomal biogenesis protein LAS1L protein (490-565AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:200-1:500 |
| Примечание | Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex, the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Ribosomal biogenesis protein LAS1L |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: LAS1L antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84, 82, 77, 34 kDa
Observed band size: 84 kDa
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Immunofluorescence staining of HepG2 cells with CSB-PA897285LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: LAS1L Antibody.