Объем | 50 мкг |
Синонимы | DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD(+)]), ligA, dnaL lig lop pdeC |
Клональность | Polyclonal Antibody |
Организм | Escherichia coli |
uniprot | P15042 |
Иммуноген | Recombinant Escherichia coli DNA ligase protein (1-671AA) |
Источник | Rabbit |
Видовая специфичность | Escherichia coli |
Применение | ELISA, WB, Recommended dilution: WB:1:1000-1:5000 |
Примечание | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Others |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: DNA ligase antibody at 2µg/ml + DH5a whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
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