Объем | 50 мкг |
Синонимы | Leucine-rich repeat flightless-interacting protein 2 (LRR FLII-interacting protein 2), LRRFIP2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9Y608 |
Иммуноген | Recombinant Human Leucine-rich repeat flightless-interacting protein 2 protein (211-342AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500 |
Примечание | May function as activator of the canonical Wnt signaling pathway, in association with DVL3, upstream of CTNNB1/beta-catenin. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Leucine-rich repeat flightless-interacting protein 2 |
Области исследований | Epigenetics and Nuclear Signaling, Immunology, Signal transduction, Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: LRRFIP2 antibody at 4.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83, 46, 12, 49, 58 kDa
Observed band size: 83, 49 kDa
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IHC image of CSB-PA896921LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA896921LA01HU diluted at 1:300 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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