DLD AntibodyСпецификация| Объем | 50 мкг | | Синонимы | Dihydrolipoyl dehydrogenase, mitochondrial (EC 1.8.1.4) (Dihydrolipoamide dehydrogenase) (Glycine cleavage system L protein), DLD, GCSL LAD PHE3 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P09622 | | Иммуноген | Recombinant Human Dihydrolipoyl dehydrogenase, mitochondrial protein (398-495AA) | | Источник | Rabbit | | Видовая специфичность | Human, Rat, Mouse | | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 | | Примечание | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). In monomeric form has additional moonlighting function as serine protease (PubMed:17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | Dihydrolipoyl dehydrogenase, mitochondrial | | Области исследований | Cancer, Metabolism, Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue, Rat brain tissue, Rat heart tissue, Mouse heart tissue
All lanes: DLD antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 44, 50 kDa
Observed band size: 55 kDa
 | IHC image of CSB-PA006928LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Информация для заказа |
Наименование: DLD Antibody.