| Объем | 100 мкг |
| Синонимы | DNA (cytosine-5)-methyltransferase 3B (Dnmt3b) (EC 2.1.1.37) (DNA methyltransferase HsaIIIB) (DNA MTase HsaIIIB) (M.HsaIIIB), DNMT3B |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UBC3 |
| Иммуноген | Recombinant Human DNA (cytosine-5)-methyltransferase 3B protein (1-100AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | DNA (cytosine-5)-methyltransferase 3B |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: DNMT3B antibody at 14µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 96, 94, 87, 81, 89, 95, 78, 82 kDa
Observed band size: 96, 37 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA883358HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA883358HA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA883358HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: DNMT3B Antibody.