| Объем | 100 мкг |
| Синонимы | Eukaryotic translation initiation factor 3 subunit M (eIF3m) (Fetal lung protein B5) (hFL-B5) (PCI domain-containing protein 1), EIF3M, HFLB5 PCID1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q7L2H7 |
| Иммуноген | Recombinant Human Eukaryotic translation initiation factor 3 subunit M protein (2-374AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Eukaryotic translation initiation factor 3 subunit M |
| Области исследований | Microbiology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat heart tissue, Rat spleen tissue
All lanes: EIF3M antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 28 kDa
Observed band size: 43 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA745333LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA745333LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA745333LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: EIF3M Antibody.