ENO1 AntibodyСпецификацияОбъем | 50 мкг | Синонимы | Alpha-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein), ENO1, ENO1L1 MBPB1 MPB1 | Клональность | Polyclonal Antibody | Организм | Human | uniprot | P06733 | Иммуноген | Recombinant Human Alpha-enolase (2-428AA) | Источник | Rabbit | Видовая специфичность | Human, Mouse | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 | Примечание | ENO1 encodes one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | >95%, Protein G purified | Области исследований | Epigenetics and Nuclear Signaling, Cancer, Metabolism, Signal transduction | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: EC109 whole cell lysate, 293T whole cell lysate
All lanes: ENO1 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 48, 37 kDa
Observed band size: 48 kDa
| Western Blot
Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse spleen tissue, Mouse lung tissue, Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue, Mouse thymus tissue
All lanes: ENO1 antibody at 2.5 µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48, 37 kDa
Observed band size: 48 kDa
| Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA02395A0Rb at dilution of 1:50
| Immunofluorescent analysis of HepG2 cells using CSB-PA02395A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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