| Объем | 50 мкг |
| Синонимы | Protein kinase C and casein kinase substrate in neurons protein 3 (SH3 domain-containing protein 6511), PACSIN3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UKS6 |
| Иммуноген | Recombinant Human Protein kinase C and casein kinase substrate in neurons protein 3 protein (1-300AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200 |
| Примечание | Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Protein kinase C and casein kinase substrate in neurons protein 3 |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat liver tissue
All lanes: PACSIN3 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA892456LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA892456LA01HU at dilution of 1:100
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Immunofluorescence staining of HepG2 cells with CSB-PA892456LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: PACSIN3 Antibody.