| Объем | 50 мкг |
| Синонимы | Peroxisome assembly protein 26 (Peroxin-26), PEX26 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q7Z412 |
| Иммуноген | Recombinant Human Peroxisome assembly protein 26 protein (1-159AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000 |
| Примечание | Probably required for protein import into peroxisomes. Anchors PEX1 and PEX6 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes. Involved in the import of catalase and proteins containing a PTS2 target sequence, but not in import of proteins with a PTS1 target sequence. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Peroxisome assembly protein 26 |
| Области исследований | Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: PEX26 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 29 kDa
Observed band size: 34 kDa
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IHC image of CSB-PA767196LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating PEX26 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA767196LA01HU in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA767196LA01HU (6µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (20µg)
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Наименование: PEX26 Antibody.