| Объем | 50 мкг |
| Синонимы | PHD finger protein 10 (BRG1-associated factor 45a) (BAF45a) (XAP135), PHF10, BAF45A |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q8WUB8 |
| Иммуноген | Recombinant Human PHD finger protein 10 protein (151-360AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: PHF10 antibody at 8µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: A2780 whole cell lysate
Lane 4: U251 whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: A375 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 57, 56, 52 kDa
Observed band size: 57 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA017898LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA017898LA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA017898LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: PHF10 Antibody.