Объем | 100 мкл |
Синонимы | C-erbB-2, ErbB2, |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P04626 |
Иммуноген | Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p)-W) derived from Human HER2. |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 |
Примечание | <p>Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin. <br /><br />Marone R, et al. (2004) Nat Cell Biol, 6(6): 515-22. <br />Ren Z, et al. (2002) J Biol Chem, 277(41): 38486-93.</p> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Tyr1221/Tyr1222 |
Области исследований | Cancer,Tags & Cell Markers,Immunology,Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MDA cells untreated or treated with EGF using HER2(Phospho-Tyr1221/Tyr1222) Antibody.
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Immunofluorescence staining of methanol-fixed MCF cells using HER2(Phospho-Tyr1221/Tyr1222) Antibody.
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Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using HER2 (Phospho-Tyr1221/Tyr1222) Antibody.
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