| Объем | 100 мкл |
| Синонимы | ER, ESR, ESR1, ESTR, ESTRA |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P03372 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 118 (Q-L-S(p)-P-F) derived from Human Estrogen Receptor-a. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Monkey |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. <br /><br />Ramsauer VP, et al. (2003)J Biol Chem, 278(32): 30142-7. <br />Jepson S, et al. (2002)Oncogene, 21(49): 7524-32. <br />Ren Z, et al. (2002)J Biol Chem, 277(41): 38486-93. <br />Eppenberger-Castori S, et al. (2001)J Clin Oncol, 19(3): 645-56.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser118 |
| Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MDA cells untreated or treated with Estradiol using Estrogen Receptor-a(Phospho-Ser118) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Phospho-Ser118) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Phospho-Ser118) Antibody.
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Western blot analysis of extracts from COS7 cells, treated with starve or calf intestinal phosphatase (CIP), using Estrogen Receptor-? (Phospho-Ser118) Antibody.
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Наименование: Phospho-ESR1 (Ser118) Antibody.