| Объем | 100 мкл |
| Синонимы | FOS, G0S7, Cellular oncogene fos, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P01100 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 232(V-A-T(p)-P-E) derived from Human FOS . |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.<br/><br/>van Straaten F. Proc. Natl. Acad. Sci. U.S.A. 80:3183-3187(1983).<br />Hai T., Genes Dev. 3:2083-2090(1989).<br />Heilig R., Nature 421:601-607(2003).</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr232 |
| Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from COS7 cells (Lane 2), using FOS (Phospho-Thr232) Antibody. The lane on the left is treated with antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Наименование: Phospho-FOS (Thr232) Antibody.