| Объем | 100 мкл |
| Синонимы | Factor A, GSK-3 alpha/beta, kinase GSK3-alpha/beta |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P49840/P49841 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3 / . |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. <br /><br />Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13, 137(1-2): 193-201 <br />Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02, 316(2): 411-415 <br />Fan G, et al. J Biol Chem 2003 Dec 26, 278(52): 52432-52436 <br />Liao X, et al. Mol Cancer Ther 2003 Nov, 2(11): 1215-1222</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr279/216 |
| Области исследований | Cardiovascular,Cell biology,Signal transduction,Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3α/β(Phospho-Tyr279/216) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α/β(Phospho-Tyr279/216) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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Immunofluorescence staining of methanol-fixed Hela cells using GSK3α/β(Phospho-Tyr279/216) Antibody.
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Immunofluorescence staining of methanol-fixed MEF cells using GSK3α/β(Phospho-Tyr279/216) Antibody.
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Наименование: Phospho-GSK3A/GSK3B (Tyr279/216) Antibody.