| Объем | 50 мкл |
| Синонимы | Histone H2AX (H2a/x) (Histone H2A.X), H2AFX, H2AX |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P16104 |
| Иммуноген | Peptide sequence around site of phospho-Ser (139) derived from Human Histone H2AX. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, ChIP, Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200 |
| Примечание | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H2AX |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
All lanes: H2AFX antibody at 1.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
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Immunofluorescence staining of Hela with CSB-PA010097OA139phHU, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Chromatin Immunoprecipitation Hela (4*106 |
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Наименование: Phospho-H2AFX (S139) Antibody.