Phospho-HIST1H3A (T3) Antibody

Спецификация

Объем	50 мкл
Синонимы	Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ
Клональность	Polyclonal Antibody
Организм	Human
uniprot	P68431
Иммуноген	Peptide sequence around site of phospho-Thr(3) derived from Human Histone H3
Источник	Rabbit
Видовая специфичность	Human, Mouse, Rat
Применение	ELISA, WB, IP, ChIP, Recommended dilution: WB:1:500-1:2000, IP:1:200-1:2000
Примечание	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Клональность1	Polyclonal
Изотип	IgG
Коньюгат	Non-conjugated
Буффер	Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Форма	Liquid
Хранение	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки	Antigen Affinity Purified
Абревеатура	Histone H3.1
Области исследований	Epigenetics and Nuclear Signaling
Ссылка на страницу на сайте производителя	ссылка
Western Blot Detected samples: Jurkat whole cell lysate, Hela whole cell lysate; Untreated (-) or treated (+) with Calyculin A and EGF All lanes: HIST1H3A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
Immunoprecipitating HIST1H3A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA010418OA03phHU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010418OA03phHU (5µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
Chromatin Immunoprecipitation Hela (4*106, treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5?g anti-HIST1H3A (CSB-PA010418OA03phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.

Информация для заказа

Область использования: Поликлональные антитела Cusabio	Производство:	Cusabio
	Метод:	Антитела
	Объем:	50 мкл
	Кат. номер:	CSB-PA010418OA03phHU
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Phospho-HIST1H3A (T3) Antibody. Примечание: дополнительная информация (на английском языке).