| Объем | 100 мкл |
| Синонимы | CK 18, CK18, CYK18, Cytokeratin endo B, K18 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P05783 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 33 (A-A-S(p)-V-Y) derived from Human Keratin 18 (CK18). |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.<br/><br/>Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.<br/>Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.<br/>Moll, R. et al. (1982) Cell 31, 11-24.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser33 |
| Области исследований | Tags & Cell Markers,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Keratin 18(Phospho-Ser33) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18(Phospho-Ser33) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-KRT18 (Ser33) Antibody.