| Объем | 100 мкл |
| Синонимы | ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P36507 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. <br/><br/>Crews C M, et al. (1992) Science. 258:478-480.<br/>Alessi D R, et al. (1994) EMBO J. 13:1610-1619.<br/>Rosen L B, et al. (1994) Neuron. 12:1207-1221.<br/>Cowley S, et al. (1994) Cell. 77:841-852.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr394 |
| Области исследований | Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HepG2 and Hela cells untreated or treated with UV using MEK-2(Phospho-Thr394) Antibody.
 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2(Phospho-Thr394) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 |
Immunofluorescence staining of methanol-fixed Hela cells using MEK-2(Phospho-Thr394) Antibody.
 |
| | |
Наименование: Phospho-MAP2K2 (Thr394) Antibody.