Объем | 100 мкл |
Синонимы | EC 2.7.11.1, kinase Mnk1, Map kinase interacting kinase, MAP kinase signal-integrating kinase 1, MAP kinase-interacting serine/threonine kinase 1, MKNK1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9BUB5 |
Иммуноген | Peptide sequence around phosphorylation site of threonine 255 (L-T-T(p)-P-C) derived from Human MNK1. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB,WB:1:500-1:3000 |
Примечание | May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Thr255 |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody. The lane on the right is treated with the synthesized peptide.
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