| Объем | 100 мкл |
| Синонимы | Serine/threonine-protein kinase PAK 3, Beta-PAK, Oligophrenin-3, p21-activated kinase 3, PAK-3, PAK3, OPHN3 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | O75914/Q13153/Q13177 |
| Иммуноген | A synthesized peptide derived from human Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Neuroscience |
| Аббревиатура | Serine/threonine-protein kinase PAK 3 |
| Примечание | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. |
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Western Blot
Positive WB detected in:A549 whole cell lysate(treated with EGF or not)
All lanes:Phospho-PAK1/PAK2/PAK3 antibody at 1.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65 KDa
Observed band size: 65 KDa
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IHC image of CSB-RA017407A144phHU diluted at 1:100 and staining in paraffin-embedded rat brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Antibody.