| Объем | 100 мкл |
| Синонимы | AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13131/P54646 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKa1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. <br /><br />Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346. <br />Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser487 |
| Области исследований | Neuroscience,Cancer,Cardiovascular,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKa1(Phospho-Ser487)Antibody.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKa1(Phospho-Ser487)Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using AMPKa1(Phospho-Ser487)Antibody.
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Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPK?1 (Phospho-Ser487) Antibody.
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Наименование: Phospho-PRKAA1/PRKAA2 (Ser487) Antibody.