| Объем | 100 мкл |
| Синонимы | PAXI, PXN, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P49023 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 |
| Примечание | An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions <br/><br/>Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140<br/>Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.<br/>Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr88 |
| Области исследований | Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Paxillin(phospho-Tyr88) Antibody.
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Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) Antibody.
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Наименование: Phospho-PXN (Tyr88) Antibody.