Объем | 100 мкл |
Синонимы | Signal transducer and activator of transcription 5B |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P51692 |
Иммуноген | Peptide sequence around phosphorylation site of serine 731 (A-P-S(p)-P-A) derived from Human STAT5B. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 |
Примечание | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser731 |
Области исследований | Epigenetics and Nuclear Signaling,Cancer,Metabolism,Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins), using STAT5B (phospho-Ser731) antibody. The lane on the right is treated with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5B (phospho-Ser731) antibody. The picture on the right is treated with the synthesized peptide.
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