PKM AntibodyСпецификация| Объем | 100 мкл | | Синонимы | PKM, PK3, OIP3, PK2, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P14618 | | Иммуноген | Peptide sequence around aa. 399~403(V-R-A-S-S) derived from Human PKM1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 | | Примечание | <p>Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. <br /><br />Christofk, H.R. et al. (2008) Nature 452, 230-3. <br />Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8. <br />Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29. <br />Hitosugi, T. et al. (2009) Sci Signal 2, ra73.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from U87 cells using PKM1 Antibody.
 | Immunofluorescence staining of methanol-fixed MEF cells using PKM1 Antibody.
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Наименование: PKM Antibody.