| Объем | 50 мкг |
| Синонимы | Proteasome subunit alpha type-1 (EC 3.4.25.1) (30 kDa prosomal protein) (PROS-30) (Macropain subunit C2) (Multicatalytic endopeptidase complex subunit C2) (Proteasome component C2) (Proteasome nu chain), PSMA1, HC2 NU PROS30 PSC2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P25786 |
| Иммуноген | Recombinant Human Proteasome subunit alpha type-1 protein (163-263AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Proteasome subunit alpha type-1 |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate
All lanes: PSMA1 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 31 kDa
Observed band size: 30 kDa
 |
Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA018865LA01HU at dilution of 1:100
 |
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA018865LA01HU at dilution of 1:100
 |
Immunofluorescent analysis of PC-3 cells using CSB-PA018865LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
 |
Immunoprecipitating PSMA1 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA018865LA01HU in K562 whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA018865LA01HU (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
 |
| | |
Наименование: PSMA1 Antibody.