Объем | 50 мкл |
Синонимы | Proteasome activator complex subunit 1 (11S regulator complex subunit alpha) (REG-alpha) (Activator of multicatalytic protease subunit 1) (Interferon gamma up-regulated I-5111 protein) (IGUP I-5111) (Proteasome activator 28 subunit alpha) (PA28a) (PA28alpha), PSME1, IFI5111 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q06323 |
Иммуноген | Recombinant Human Proteasome activator complex subunit 1 protein (1-249AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Proteasome activator complex subunit 1 |
Области исследований | Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: PSME1 antibody at 0.75µg/ml
Lane 1: Mouse liver tissue
Lane 2: Raji whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29, 27 kDa
Observed band size: 29 kDa
![](http://www.cusabio.cn/images/antibody/2/CSB-PA018915DSR1HU-WB.jpg) |
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA018915DSR1HU at dilution of 1:100
![](http://www.cusabio.cn/images/antibody/2/CSB-PA018915DSR1HU-IHC-1.jpg) |
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA018915DSR1HU at dilution of 1:100
![](http://www.cusabio.cn/images/antibody/2/CSB-PA018915DSR1HU-IHC-2.jpg) |
Immunoprecipitating PSME1 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA018915DSR1HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA018915DSR1HU (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)
![](http://www.cusabio.cn/images/antibody/4/CSB-PA018915DSR1HU-IP.jpg) |
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