Объем | 50 мкг |
Синонимы | Prostaglandin reductase 2 (PRG-2) (EC 1.3.1.48) (15-oxoprostaglandin 13-reductase) (Zinc-binding alcohol dehydrogenase domain-containing protein 1), PTGR2, ZADH1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q8N8N7 |
Иммуноген | Recombinant Human Prostaglandin reductase 2 protein (206-282AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Prostaglandin reductase 2 |
Области исследований | Cell biology, Metabolism |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate
All lanes: PTGR2 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 20 kDa
Observed band size: 39 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA018984LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA018984LA01HU at dilution of 1:100
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Immunofluorescence staining of A549 cells with CSB-PA018984LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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