Объем | 50 мкг |
Синонимы | Parvalbumin alpha, PVALB |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P20472 |
Иммуноген | Recombinant Human Parvalbumin alpha protein (2-110AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Parvalbumin alpha protein |
Области исследований | Neuroscience |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Parvalbumin alpha antibody at 2µg/ml + Mouse skeletal muscle tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA019092HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA019092HA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: mouse brain tissue, Mouse kidney tissue
All lanes: PVALB antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
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Immunofluorescent analysis of HepG2 cells using CSB-PA019092HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA019092HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA019092HA01HU at dilution of 1:100
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Immunofluorescence staining of U251 cells with CSB-PA019092HA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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