| Объем | 50 мкг |
| Синонимы | UDP-glucose 4-epimerase (EC 5.1.3.2) (Galactowaldenase) (UDP-N-acetylgalactosamine 4-epimerase) (UDP-GalNAc 4-epimerase) (UDP-N-acetylglucosamine 4-epimerase) (UDP-GlcNAc 4-epimerase) (EC 5.1.3.7) (UDP-galactose 4-epimerase), GALE |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14376 |
| Иммуноген | Recombinant Human UDP-glucose 4-epimerase protein (1-348AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:1000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: UDP-glucose 4-epimerase antibody at 2µg/ml
Lane 1: HepG2 cells
Lane 2: Hela cells
Lane 3: A549 cells
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39, 31 kDa
Observed band size: 39 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA613499HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA613499HA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA613499HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: GALE Antibody.