| Объем | 100 мкг |
| Синонимы | Transcriptional repressor p66-alpha (Hp66alpha) (GATA zinc finger domain-containing protein 2A), GATAD2A |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q86YP4 |
| Иммуноген | Recombinant Human Transcriptional repressor p66-alpha protein (170-241AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000 |
| Примечание | Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Transcriptional repressor p66-alpha |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, Rat stomach tissue
All lanes: GATAD2A antibody at 2.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 69, 66 kDa
Observed band size: 69 kDa
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IHC image of CSB-PA803156LA01HU diluted at 1:800 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA803156LA01HU diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: GATAD2A Antibody.