| Объем | 100 мкл |
| Синонимы | P105-RB, PP105, PP110, RB-1, RB1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P06400 |
| Иммуноген | Peptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 |
| Примечание | Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter <br/><br/>Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.<br/>Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.<br/>Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.<br/>Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from K562 cells using Rb(Ab-807) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Ab-807) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: RB1 (Ab-807) Antibody.