RELA (Ab-254) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | NFKB3, RELA, TF65, Transcription factor p65, p65 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q04206 | | Иммуноген | Peptide sequence around aa.252~256 (F-R-T-P-P) derived from Human NFkB-p65. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, IHC,IHC:1:50-1:100 | | Примечание | NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex<br/><br/>Yeh PY, et al. (2004) J Biol Chem. 279(25): 26143-26148.<br/>Ryo A, et al. (2003) Mol Cell.12 (6): 1413-1426.<br/>Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.<br/>Baeuerle P A, et al. (1996) Cell 87:13-20.<br/>Haskill S, et al. (1991) Cell 65:1281-1289.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Cardiovascular,Cell biology,Metabolism,Microbiology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p65(Ab-254) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: RELA (Ab-254) Antibody.