| Объем | 50 мкг |
| Синонимы | Replication protein A 14 kDa subunit (RP-A p14) (Replication factor A protein 3) (RF-A protein 3), RPA3, REPA3 RPA14 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P35244 |
| Иммуноген | Recombinant Human Replication protein A 14 kDa subunit protein (1-119AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, Recommended dilution: WB:1:1000-1:5000 |
| Примечание | Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Ref.7 Ref.9 Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair, it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Replication protein A 14 kDa
subunit antibody at 2µg/ml
Lane 1: LO2 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
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Наименование: RPA3 Antibody.