Объем | 100 мкг |
Синонимы | Protein S100-A4 (Calvasculin) (Metastasin) (Placental calcium-binding protein) (Protein Mts1) (S100 calcium-binding protein A4), S100A4, CAPL MTS1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P26447 |
Иммуноген | Recombinant Human Protein S100-A4 protein (2-101AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:1000, IHC:1:20-1:200 |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cancer, Cardiovascular, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Protein S100-A4 antibody at 2µg/ml + A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA020632HA01HU at dilution of 1:100
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IHC image of CSB-PA020632HA01HU diluted at 1:600 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA020632HA01HU diluted at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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