| Объем | 100 мкг |
| Синонимы | Semaphorin-5B, SEMA5B, KIAA1445 SEMAG |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9P283 |
| Иммуноген | Recombinant Human Semaphorin-5B protein (845-1013AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200 |
| Примечание | May act as positive axonal guidance cues. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Semaphorin-5B |
| Области исследований | Neuroscience, Developmental biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse heart tissue
All lanes: SEMA5B antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 126, 120, 124, 132 kDa
Observed band size: 126 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA868389LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA868389LA01HU at dilution of 1:100
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Immunofluorescence staining of Hela cells with CSB-PA868389LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: SEMA5B Antibody.