SLC25A24 AntibodyСпецификация| Объем | 100 мкг | | Синонимы | Calcium-binding mitochondrial carrier protein SCaMC-1 (Mitochondrial ATP-Mg/Pi carrier protein 1) (Mitochondrial Ca(2+)-dependent solute carrier protein 1) (Small calcium-binding mitochondrial carrier protein 1) (Solute carrier family 25 member 24), SLC25A24, APC1 MCSC1 SCAMC1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q6NUK1 | | Иммуноген | Recombinant Human Calcium-binding mitochondrial carrier protein SCaMC-1 protein (1-197AA) | | Источник | Rabbit | | Видовая специфичность | Human, Rat, Mouse | | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 | | Примечание | Calcium-dependent mitochondrial solute carrier. Mediates the reversible, electroneutral exchange of Mg-ATP or Mg-ADP against phosphate ions, catalyzing the net uptake or efflux of adenine nucleotides across the mitochondrial inner membrane. Nucleotide transport is inactive when cytosolic calcium levels are low, and is activated by an increase in cytosolic calcium levels. May play a role in protecting cells against oxidative stress-induced cell death, probably by promoting the formation of calcium-phosphate precipitates in the mitochondrial matrix, and thereby buffering calcium levels in the mitochondrial matrix. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | Calcium-binding mitochondrial carrier protein SCaMC-1 | | Области исследований | Metabolism, Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, Rat kidney tissue, Mouse heart tissue
All lanes: SLC25A24 antibody at 5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54, 52 kDa
Observed band size: 54 kDa
 | IHC image of CSB-PA743993LA01HU diluted at 1:250 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of U251 cells with CSB-PA743993LA01HU at 1:110, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
 | Western Blot
Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, U87 whole cell lysate, K562 whole cell lysate
All lanes: SLC25A24 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54, 52 kDa
Observed band size: 54 kDa
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Информация для заказа |
Наименование: SLC25A24 Antibody.