| Объем | 100 мкг |
| Синонимы | Sorting nexin-12, SNX12 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UMY4 |
| Иммуноген | Recombinant Human Sorting nexin-12 protein (2-172AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500, IP:1:200-1:2000 |
| Примечание | May be involved in several stages of intracellular trafficking. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Sorting nexin-12 |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA891787LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of A549 cells with CSB-PA891787LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Western Blot
Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate
All lanes: SNX12 antibody at 2.83µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20, 19 kDa
Observed band size: 20 kDa
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Immunoprecipitating SNX12 in A549 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA891787LA01HU in A549 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA891787LA01HU (6µg) + A549 whole cell lysate (500µg)
Lane 3: A549 whole cell lysate (20µg)
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Наименование: SNX12 Antibody.