| Объем | 50 мкл |
| Синонимы | Spindlin-1 (Ovarian cancer-related protein) (Spindlin1), SPIN1, OCR SPIN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9Y657 |
| Иммуноген | Recombinant Human Spindlin-1 protein (1-262AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway dowstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed:24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed:21960006). May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Spindlin-1 |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Developmental biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: SPIN1 antibody at 1.45µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA897305DSR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA897305DSR1HU at dilution of 1:100
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Наименование: SPIN1 Antibody.