| Объем | 50 мкг |
| Синонимы | Serine palmitoyltransferase 3 (EC 2.3.1.50) (Long chain base biosynthesis protein 2b) (LCB2b) (Long chain base biosynthesis protein 3) (LCB 3) (Serine-palmitoyl-CoA transferase 3) (SPT 3), SPTLC3, C20orf38 SPTLC2L |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9NUV7 |
| Иммуноген | Recombinant Human Serine palmitoyltransferase 3 protein (1-58AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Примечание | Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: SPTLC3 antibody at 0.2µg/ml
Lane 1: Mouse liver tissue
Lane 2: Mouse kidney tissue
Lane 3: U87 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 63, 20 kDa
Observed band size: 63 kDa
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Immunofluorescent analysis of HepG2 cells using CSB-PA865142LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, Rat heart tissue, Rat kidney tissue
All lanes: SPTLC3 antibody at 4.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63, 20 kDa
Observed band size: 63 kDa
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IHC image of CSB-PA865142LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: SPTLC3 Antibody.