| Объем | 100 мкг |
| Синонимы | Nucleoporin GLE1 (hGLE1) (GLE1-like protein), GLE1, GLE1L |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q53GS7 |
| Иммуноген | Recombinant Human Nucleoporin GLE1 protein (141-380AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate
All lanes: GLE1 antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 80, 76 kDa
Observed band size: 80 kDa
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Immunocytochemistry analysis of human melanoma using CSB-PA700651EA01HU at dilution of 1:100
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Immunocytochemistry analysis of human breast cancer using CSB-PA700651EA01HU at dilution of 1:100
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Immunofluorescent analysis of MCF-7 cells using CSB-PA700651EA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating GLE1 in A549 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA700651EA01HU in A549 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA700651EA01HU (6µg) + A549 whole cell lysate (500µg)
Lane 3: A549 whole cell lysate (20µg)
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Наименование: GLE1 Antibody.