| Объем | 100 мкг |
| Синонимы | Synaptoporin, SYNPR |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q8TBG9 |
| Иммуноген | Recombinant Human Synaptoporin protein (199-265AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: SYNPR antibody at 0.2µg/ml + Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 30, 32 kDa
Observed band size: 30 kDa
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Immunofluorescent analysis of U87 cells using CSB-PA023023LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: Rat lung tissue
All lanes: SYNPR antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 32 kDa
Observed band size: 30 kDa
 |
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA023023LA01HU at dilution of 1:100
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Immunofluorescence staining of U251 cells with CSB-PA023023LA01HU at 1:145, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescence staining of MCF-7 cells with CSB-PA023023LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: SYNPR Antibody.