Объем | 100 мкл |
Синонимы | SYT1/2, SytI/II, Synaptotagmin I/II, P65, SYT |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P21579/Q8N9I0 |
Иммуноген | Peptide sequence around aa.200~204/197~201 (R-K-T-L-N) derived from Human Synaptotagmin 1/2. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 |
Примечание | <p>The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse <br /><br />Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11, 105(10):3992-7. <br />Cnops L, et al. Cereb Cortex. 2008 May, 18(5):1221-31. <br />Lynch KL, et al. Mol Biol Cell. 2007 Dec, 18(12):4957-68.</p> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
Области исследований | Neuroscience,Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells using Synaptotagmin 1/2(Ab-202/199) Antibody and the same antibody preincubated with blocking peptide.
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Immunofluorescence staining of methanol-fixed Hela cells using Synaptotagmin 1/2(Ab-202/199) Antibody.
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