| Объем | 50 мкг |
| Синонимы | Tubulin-folding cofactor B (Cytoskeleton-associated protein 1) (Cytoskeleton-associated protein CKAPI) (Tubulin-specific chaperone B), TBCB, CG22 CKAP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q99426 |
| Иммуноген | Recombinant Human Tubulin-folding cofactor B protein (1-244AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: TBCB antibody at 4µg/ml
Lane 1: Hela whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Mouse thymus tissue
Lane 4: A549 whole cell lysate
Lane 5: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28, 22 kDa
Observed band size: 28 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA03169A0Rb at dilution of 1:100
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Immunoprecipitating TBCB in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA03169A0Rb in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA03169A0Rb (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Immunofluorescent analysis of Hela cells using CSB-PA03169A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: TBCB Antibody.