| Объем | 50 мкг |
| Синонимы | Ganglioside GM2 activator (Cerebroside sulfate activator protein) (GM2-AP) (Sphingolipid activator protein 3) (SAP-3) [Cleaved into: Ganglioside GM2 activator isoform short], GM2A |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P17900 |
| Иммуноген | Recombinant Human Ganglioside GM2 activator protein (32-193AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Ganglioside GM2 activator |
| Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Ganglioside GM2 activator antibody at 10µg/ml
Lane 1: Rat liver tissue
Lane 2: Mouse stomach tissue
Lane 3: Mouse small intestine tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA02379A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA02379A0Rb at dilution of 1:100
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Immunoprecipitating GM2A in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA02379A0Rb in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA02379A0Rb (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)
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Наименование: GM2A Antibody.