Объем | 100 мкг |
Синонимы | DNA topoisomerase 2-beta (EC 5.99.1.3) (DNA topoisomerase II, beta isozyme), TOP2B |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q02880 |
Иммуноген | Recombinant Human DNA topoisomerase 2-beta protein (1400-1626AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | DNA topoisomerase 2-beta |
Области исследований | Epigenetics and Nuclear Signaling, Cancer |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA717547LA01HU at dilution of 1:100
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Immunofluorescence staining of Hela cells with CSB-PA717547LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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