| Объем | 50 мкг |
| Клон ID | 2A8D2 |
| Синонимы | Glutathione S-Transferase, gst, GST tag |
| Тип антител | Tag/Control Antibodies |
| Иммуноген | Recombinant GST Protein |
| Источник | Mouse |
| Применение | ELISA,WB,Recommended dilution:WB:1:500-1:5000 |
| Изотип | IgG2a |
| Коньюгат | Non-conjugated |
| Метод очистки | >95%,Protein G purified |
| Буффер | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Примечание | Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. |
| Ссылка на страницу на сайте производителя | ссылка |
All lanes : Recombinant GST Protein at 30ng/ml
Line 1:Mouse Anti-GST monoclonal antibody at 1:1000
Line2:Mouse Anti-GST monoclonal antibody at 1:2000
Line3:Mouse Anti-GST monoclonal antibody at 1:5000
Line4:Mouse Anti-GST monoclonal antibody at 1:10000
Line5:Mouse Anti-GST monoclonal antibody at 1:20000
Line6:Mouse Anti-GST monoclonal antibody at 1:40000
Line7:Mouse Anti-GST monoclonal antibody at 1:80000
Predicted band size : 28kd
Observed band size : 30kd
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Наименование: GST Monoclonal Antibody.