HDAC3 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Histone deacetylase 3, HD3, RPD3-2, SMAP45, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | O15379 | | Иммуноген | Synthesized peptide derived from C-terminal of human HDAC3. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1, increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.<br/><br/>Xiaohong Zhang, Genes & Dev., Apr 2005, 19: 827 - 839.<br/>Zhanguo Gao, J. Biol. Chem., Feb 2006, 281: 4540 - 4547. Andrew J. Wilson, J. Biol. Chem., May 2006, 281: 13548 - 13558.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cardiovascular,Metabolism,Stem cells | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC3 antibody.
 | Immunofluorescence analysis of COS7 cells, using HDAC3 antibody.
 | Western blot analysis of extracts from HepG2 cells, using HDAC3 antibody.
 | Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using HDAC3 antiobdy. The lane on the left is treated with systhesized peptide.
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Наименование: HDAC3 Antibody.