HIST1H3A (Ab-4) AntibodyСпецификацияОбъем | 50 мкл | Синонимы | Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ | Клональность | Polyclonal Antibody | Организм | Human | uniprot | P68431 | Иммуноген | Peptide sequence around site of Lys (4) derived from Human Histone H3.1. | Источник | Rabbit | Видовая специфичность | Human, Mouse, Rat | Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | Antigen Affinity Purified | Абревеатура | Histone H3.1 | Области исследований | Epigenetics and Nuclear Signaling | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: Hela cell acid extracts, Jurkat cell acid extracts, HEK293 cell acid extracts, Mouse kidney tissue
All lanes: HIST1H3A antibody at 0.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
| Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010418NA04nme3HU at dilution of 1:100
| Immunofluorescent analysis of Hela cells using CSB-PA010418NA04nme3HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
| Immunoprecipitating HIST1H3A in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010418NA04nme3HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010418NA04nme3HU (5µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Информация для заказаОбласть использования: | Производство: | Cusabio | Метод: | Антитела | Объем: | 50 мкл | Кат. номер: | CSB-PA010418NA04nme3HU | Цена (с НДС 20%): | по запросу | В корзину | Наименование: HIST1H3A (Ab-4) Antibody. Примечание: дополнительная информация (на английском языке). |
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